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polyacrylamide gel electrophoresis of DNA

a. Quick review of the steps in making a polyacrylamide gel.
b. What polyacrylamide gel electrophoresis enables the scientist to do that agarose gel electrophoresis doesn’t, with regard to analyzing DNA.
c. Describe the two applications of polyacrylamide gel electrophoresis: slab gel electrophoresis and capillary electrophoresis.
d. Present the step by step analysis of a slab or capillary electrophoresis result.

2. Write five multiple choice/short answer questions that would assess a student’s understanding of your presentation on polyacrylamide gel electrophoresis.

3. Propose a hypothesis that could be directly tested using polyacrylamide gel electrophoresis. What would the experimental and control groups be? What results would you expect if your hypothesis was supported?

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